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KMID : 0545120040140051071
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 5 p.1071 ~ p.1074
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Cold Adaptation of Lactobacillus paraplantarum C7 Isolated from Kimchi
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Ha YL
Kim SJ/Kim JH/Park JY/Kim HT/Jeong SJ/Yun HD/Kim JH
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Abstract
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The effect of preadaptation at low temperature cryoprotection was studied for Lactobacillus paraplantarum C7 a bacteriocin producer isolated from kimchi. L. paraplantarum C7 cells in their log growth phase were incubated at 15o 10oC or 5oC for 2 4 and 6 h respectively before being frozen at -70oC. After 24 h of freezing viable cells were counted after brief thawing. The freezing-thawing cycles were repeated three more times. Cells preadapted at 10oC or 5oC before freezing survived better than control cells but preadaptation at 15oC did not confer cryoprotection. Chloramphenicol addition did not destroy the cryoprotection indicating that protein synthesis was not required for the development of cryoprotection. SDS-PAGE showed induction of a 6.5-kDa protein a major cold-shock protein in preadapted cells.
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